BI 171 - Third Exam - 2003

Answer Key

 

Multiple Choice.

Place the letter of the choice that best answers the question on the line to the left.
Two Points Each. NOTE: "e" answers are never the correct answer.

 

___B___ 1. The 9 + 2 pattern involves

                    a. Cell connections in multicelled systems                     b. Microtubules
   
                     c. Particle exchange through a membrane                      d. Chromosomes
   
                                                                 e. Ummmm, eleven-?

                        ...it's the arrangement in cilia and flagella.

 

___C___ 2. Which are the reactions in coupled reactions?

                    a. Hydrolysis & dehydration synthesis
   
                     b. Oxygen-producing & oxygen-using
   
                     c. Endergonic & exergonic
                    d. Enzyme-catalyzed & non-enzyme-catalyzed
   
                     e. That’s really no one’s business but their’s

                        ...the endergonic reactions pick up their energy from the
                            exergonic ones.

 

___D___ 3. A ligand can also be called a

                    a. Chaperonin                     b. Domain                     c. Channel
                    d. Substrate                                 e. Suitably nasty name

                        ...it's a molecule that binds to some protein region, like a substrate
                            binds to an enzyme's active site.

 

___A___ 4. The plant cells in the lab exercise, in fresh water, are surrounded by which 
                                type of
solution?
                    a. Hypotonic                     b. Hypertonic                     c. Isotonic
   
                     d. Cytotonic                                     e. Toonytonic

                        ...fresh water, with almost no dissolved materials, had much more
                            water and less "stuff."

 

___C___5. A protein other than enzymes that follows Michaelis-Menten patterns:

                     a. Microtubules                     b. Histones                    c. Carriers 
                    d. Centromeres   
                                     e. Annoying ones

                        ...they follow that "worker" curve - give them more to carry, and they
                            carry more (the movement rate goes up), until the molecules are active
                            100% of the time and can't (the movement rate stops increasing).

 

___D___6. Introns are

                    a. Materials released from a cell
   
                     b. Parts of an enzyme that are not functional
   
                     c. Byproducts of an enzyme-catalyzed reaction
   
                     d. Parts of a gene sequence that are not used in the coded protein
   
                     e. Some sort of big honkin’ corporate monster

                        ...from beginning to end of a gene, not all of the sequence is used as
                            code - the used sequence is the exon.

 

___C___7. A cell membrane’s fluidity is affected by

                        a. Phosphate groups                 b. Carriers                 c. Cholesterol
                   d. Flux                                                 e. How drunk it is

                        ...these little molecules in the hydrophobic layer act like lubricants.

 

___A___8. Which is a critical protein in microfilaments?

                    a. Actin     b. Dynein      c. Thylakoid      d. Kinesin     e. Filamentin

                        ...it is the most common microfilament component.

 

___B___9. Which are, on a molecular level, most analogous to spools?

                    a. Plasmids                     b. Histones                     c. Microtubules
                    d. Microfilaments                         e. Twirlyloopidies

                        ...they are proteins that DNA wraps around.

 

___D___10. Which process has its first steps occur in the cytoplasm?

                     a. Photosynthesis                         b. DNA transcription  
                    c. RNA production                       d. Aerobic respiration
   
                                                 e. Training camp

                        ...a happens all in chloroplasts, b and c in the nucleus, while d
                            ends
in mitochondria.

 

___B___11. Which are most likely to be covered by microvilli?

                    a. Breathing tube cells moving mucus
   
                     b. Kidney tube cells moving wastes into urine
   
                     c. Protective skin cells
   
                     d. Cells inside a mitochondrion
   
                     e. The typical cafeteria lunch

                        ...it has to be an example where materials move through a    
                            surface, which microvilli add to.

 

___D___12. If the products of a reaction are continually removed, the reaction will 
                                reach
equilibrium

                    a. Faster                 b. Slower                 c. At the same rate
   
                     d. Never                             e. Next Tuesday

                        ...as long as the products are removed, there's nothing to change in
                            reverse, which is necessary for equilibrium.

 

___C___13. Which is counteracted by gravity?

                    a. Turgor pressure             b. Telomerase             c. Root pressure
                    d. Optimum pH                             e. Spandex

                        ...it pushes water up a plant until gravity stops it.

 

___C___14. An enzyme with a low Michaelis constant has high affinity, which means it

                    a. Works with multiple substrates                 b. Is limited in substrate choice
                    c. Works more slowly                                 d. Works more quickly
   
                                                             e. A fin’ lookin’ enzyme

                        ...affinity is strength of binding - high affinity means the enzyme grabs
                            the substrate quickly but holds it tighter, is slower to release the products
                            - a low constant occurs on a very steep curve that tops out quickly.

 

___C___15. Waterproof body coverings probably first evolved as an adaptation to

                        a. Land environments                        b. Salty marsh environments
                    c. Fresh water environments             d. Deep ocean environments
   
                                                             e. Matching boots

                        ...the barriers were to prevent osmosis from the dilute surroundings into the
                            tissues.

 

___A___16. Which would most likely involve glycocalyx?

                    a. Recognition between cells in multicelled systems
   
                     b. Movement of a cell across a surface
   
                     c. Movement of a cell by swimming
   
                     d. Connections between cells in multicelled systems
   
                     e. An alphabet chart and a handful of darts

                        ...this is commonly used as markers on cells.

 

___C___17. A Q10 compares a reaction’s rate with the reaction under what changed condition?

                        a. Ten times more reactant                         b. Ten times less reactant
                   c. 10o C rise in temperature                        d. 10o C drop in temperature
   
                                 e. Ten times more colorful gay guys criticizing it

                        ...connect the symbol to the concept.

 

___B___18. Group translocation involves what two processes?

                    a. Transport in and transport out
   
                     b. Transport and chemical change
   
                     c. Removal of one organic group and replacement by another
   
                     d. Movement of genetic materials between two cells
   
                     e. Confusion and induction of anger

                        ...match term and definition.

 

Short Answer. 

Pick NINE questions to answer in the spaces provided.
NOTE
: if you answer MORE than nine, only the first nine will be corrected.
Four Points each. Partial credit is possible.

1. What are two different types of molecules, associated with enzymes, contain minerals?
Cofactors Prosthetic groups
2. How exactly does a phospholipid molecule from a cell membrane differ from a "typical" lipid molecule?

      ...there is a phosphate group where one of the fatty acids would be attached to the glycerol in a typical lipid.

3. Briefly explain the reasoning behind how a protein or DNA sequence can be used as a molecular clock.

      ...a sequential molecule passed from a shared ancestor accumulates random mutation changes along the separate family lines - the farther back in time the shared ancestor, the more different the compared sequences should be.

 

4. Give a complete definition for turnover number.

      ...it's the number of substrate molecules processed by an enzyme molecule each second.

 

5. A cell with a lot of Golgi bodies in it would probably be doing what?

      ...producing secretions.

 

6. What are the two attachment ends of transfer RNA typically attached to?
messenger RNA  1 amino acid
7. Name or describe two different types of Microtubule-Organizing Centers.
Centrosomes (produce spindle fibers for cell division) Basal Bodies (produce microtubule core of cilia / flagella)
8. What are two different general features of active transport?
They use energy. They move things from high concentrations to low (opposite to diffusion).
9. Particles may be excluded from cell membrane pores according to what two features?
Size Charge
10. Which two organelles have internal membranes and their own DNA?
Mitochondria Chloroplasts
11. By what mechanism do prokaryotes come closest to the genetic recombination of sexual reproduction?

      ...passing of plasmids between cells.

12. Name two different types of plastids.
Chloroplasts.
Leukoplasts.
Chromoplasts.
13. Given that the "barrier" is permeable to everything, show how the various materials should flux between the boxes
60% Water                <--------------

20% Sodium              <--------------

20% Glucose             --------------->

70% Water

25% Sodium

5% Glucose

14. Name two structures associated fairly exclusively with the nucleus.
Chromosomes. Nucleolus.
Nuclear Envelope.
15. Using the table attached to the exam (detach it if you need to), translate this gene sequence into an amino acid sequence -

    T  A  C / G  G  T / A  G  G / C  G  A / A  G  G / C  C  C / G  G  T / A  C  T
codons - AUG - CCA - UCC - GCA - UCC - GGG - CCA - UGA

                      (start) - proline- serine - alanine - serine - glycine - proline - (stop)

 

 

Long Answer. 

Select and answer completely any four of the following questions.
Note:
if you answer more than four, only the first four will be corrected.
Seven Points Each. Partial credit is possible.

1. Give three sets of differences between -

CILIA

FLAGELLA

Smaller Larger
Always numerous on cell Rarely more than 12 on cell
Don't have added structures Sometimes have added structures
Tend to stroke Tend to spin
2. Briefly explain three different approaches used in the inhibition of enzymes.
Binding in the active site.
Binding near active site and blocking substrate access.
Binding to enzyme & changing its shape, including shape of active site.
Restricting one or more substrates from enzyme area.
3. Name and give the basic function of three different types of vesicles or vacuoles.
Food Vacuoles Site of digestion
Lysosomes Contain digestive enzymes for food vacuoles or cell death
Central Vacuoles Pressurized water gives structure to some plant cells
Peroxisomes Contain metabolic processes that require isolation;  site of molecule breakdown for recycling.
Contractile Vacuoles Pump out water flowing in by osmosis
4. Briefly describe four ways that flux can be increased through a barrier.
Reduce particle size Increase concentration gradient (add to high side and/or subtract from low side)
Increase temperature Increase surface area of barrier
5. In the graph below of Michaelis-Menten Kinetics, explain why the curve is doing what it’s doing at each marked point, in terms of the enzymes at work.
 

                                                                     All enzymes are active all the time, can't work any faster
                                                                  * * * * * X * * * * * * * * * * * * * * * * * * * * * * * * * * 
            Reaction                                X
                                                        *         Enzyme molecules are being overwhelmed with work, becoming less
              Rate                                *                  available as more substrate added.    
                                                     *
               V                                  *       As more substrate added, available enzymes quickly help it change -  
                                                   X       the more you add, the faster the reaction rate      
                                         _____________________________________________________________
                                                    
Substrate Concentration (as Enzymes remain constant) ---->

6. When enzyme activity on reaction rate is graphed by temperature, the rate declines on each side of an optimum. As temperature moves away from the optimum, explain what happens on a molecular level to makes the rate decline -
Below the optimum,            Molecules move / react more slowly, and the enzymes may
as the temperature               contract, becoming less- than- working shapes.
drops?
Above the optimum,            Enzymes tend to shake and unravel, leaving fewer and fewer
as the temperature              (as it gets warmer) shaped correctly.

rises?
7. Fill in the labels on this drawing of eukaryote cell structures.

This would have been a simple line drawing with several cell structures indicated.

 

 

 

 

 

 

 

NO KEY FOR  BONUS QUESTIONS. 

Answer as many or as few as you wish. You can't lose points on the rest of the exam by getting these wrong. Partial credit is possible.

 

Name the general terms given to the various proteins that contribute to DNA translation. Three Points each.

 

 

Briefly explain how one codon can stand for two different things. Four Points.

 

 

Enzyme-catalyzed reactions are not reversible through the enzymes. Why? Four Points.

 

 

What is the typical limitation of the term vitamin? Three Points.

 

 

Some enzymes have a broad, low curve for their temperature - activity graph. Why? Four Points.

 

 

Cancer cells develop two capabilities that most normal cells, including the original cells that became cancerous, do not have. For Three Points each, what are they?

 

 

Which Kingdom of organisms do not have cell walls? Three Points.

 

 

Where do steroid hormones usually connect on / in a cell? Four Points.

 

 

GENE TRANSLATION TABLE

CODONS ON MESSENGER RNA

First

Letter

SECOND

LETTER

Third

Letter

U

C

A

G

phelyalanine

serine

tyrosine

cysteine

U

U

phelyalanine

serine

tyrosine

cysteine

C

leucine

serine

STOP

STOP

A

leucine

serine

STOP

tryptophan

G

leucine

proline

histidine

arginine

U

C

leucine

proline

histidine

arginine

C

leucine

proline

glutamine

arginine

A

leucine

proline

glutamine

arginine

G

isoleucine

threonine

asparagine

serine

U

A

isoleucine

threonine

asparagine

serine

C

isoleucine

threonine

lysine

arginine

A

*START*
methionine

threonine

lysine

arginine

G

valine

alanine

aspartate

glycine

U

G

valine

alanine

aspartate

glycine

C

valine

alanine

glutamate

glycine

A

valine

alanine

glutamate

glycine

G

 

It’s okay to tear this sheet off the exam.

BI 171

McDarby

 

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