SC 135 - Third Exam Fall 2009

MULTIPLE CHOICE.

On the line to the left, place the letter of the choice that best answers the question.
Three Points Each. NOTE: "e" answers are never the correct answer.


                1.  A karyotype involves

___C___ a. Comparing cell shapes            b. Counting cell divisions            c. Comparing chromosome shapes            d. Counting organelles
                                                                                e. Folks that look like Kary

                     ...it's the comparison of overall numbers and shapes that's unique to each species.



                2.  Genetic redundancy applies to

___A___ a. Crossing over and substitution mutations            b. Enzyme amplification and meiosis II            c. Allele amplification and meiosis I
              d. Prokaryotes only                                                                            e. What terms I've totally forgotten

                     ...redundancy from extra gene copies in the first, redundancy from mutations giving the same amino acids in the second.



                3.  In mosaicism, cells have different

___D___ a. Parents             b. Numbers of nuclei            c. Types of projections             d. Numbers of chromosomes            e. Religions

                     ...they weren't distributed properly during a cell division, putting one too many in one (and its descendants), one too few
                       in the other.



                4.  In the lab, our plant cells shrank in the heavy salt solution due to

___C___ a. Osmosis into the cells             b. Attraction to salt            c. Osmosis out of the cells             d. Diffusion of salt
                                                    e. They were very well trained ahead of time

                     ...shrinking implies loss, and water diffused (osmosis) from where there was more (inside) to where there was less (the heavily
                        salty solution outside).



                5.  Much of a cell's RNA is stored in

___D___ a. Chromosomes             b. Ribosomes            c. Endoplasmic reticulum             d. Nucleoli            e. In little plastic tubs with snap lids

                     ...it's one of the basic functions.



                6.  Which vesicle would have digestive enzymes in it?

___A___ a. Lysosome             b. Ribosome            c. Digestisome             d. Nucleosome            e. Tum-tum-osome

                     ...again, the basic function.



                7.   A zygote is ________ and divides by ________

___C___ a. Haploid...meiosis             b. Haploid...mitosis            c. Diploid...meiosis             d. Diploid...mitosis            e. A weird goat...splitting

                     ...2 sets of chromosomes, and makes copies of itself.



                8.  There is a much higher concentration of iodine inside thyroid cells than in the fluid around them. Iodine moves into the cells by

___B___ a. Cilia             b. Active transport             c. Osmosis            d. Passive transport             e. Skateboard

                     ...if it moved passively by diffusion, it would go the other way.




                9.   Position effects influence how

___B___ a. Quickly cells divide             b. Easily genes get expressed            c. Organelles work together             d. All of these
                                                                e. Well, if you want to make boy babies...

                     ...some chromosome positions are more densely bundled, making genes there harder to get at and use.



                10.  The chromosome number of prokaryotes is

___C___ a. Always divisible by two             b. Always divisible by three            c. One             d. Not reliable            e. Always higher than they want to admit

                     ...they just have the one, loop-shaped chromosome.


 


                11. Radiation is most likely to damage cells that are

___D___ a. Small                 b. Big                 c. Old                d. Dividing                 e. Just being wusses

                     ...that's when DNA breaks, bad repairs, and loose pieces become important.




                12.  Crossing over typically happens during

___B___ a. Interphase             b. Meiosis I             c. Meiosis II            d. Mitosis             e. The middle of a moonless night

                     ...that's when homologous chromosomes are pulled in next to each other.





SHORT ANSWER.

Answer any eight of the following questions for 4 Points Each.
Note:
if you answer more than eight, only the first eight will be corrected.
You can get partial credit on these answers.

1.  What is the function of chaperonins?

               ...they make sure newly-made proteins take on their proper working shape.
2. For polyploidy -
What
is it?      Extra sets of chromosomes.
What types
of organisms       Plants, since it often doesn't kill them
is it found in?         (it almost always kills animals)
 
3.  What is apoptosis?


               ...cells die because they're supposed to / cell suicide.


4.  Put these in organizational order - organs, tissues, cells
1   Cells 2   Tissues 3   Organs
5.  For a low chromosome number (compared to a high one):
ADVANTAGE:    Fewer mistakes get made distributing chromosomes
                                during cell division.

DISADVANTAGE:  Fewer ways to mix them produces less variety
                                    in offspring.
6.  Define: what is diffusion?

               ...net movement of particles from a high-concentration area to a low-concentration area.

7.  What are two different things that happen during meiosis that don't happen during mitosis?
Homologous chromosomes are placed together.

Four cells are made.
 

Two divisions must happen.

Homologous chromosomes are separated.

Cells from first stage are haploid.

8.  What organelles probably started as endosymbionts?

Chloroplasts
 

Mitochondria
9.  Why is a deletion mutation much more dangerous than a substitution mutation?   Give a bit of explanation.

               ...a substitution changes one letter in one codon, but a deletion shifts the end of that codon and every one that follows one spot up.



10.  Give the functions (not just the names) of two different types of vacuoles.

Hold & Digest Food (Food Vacuole)

Pump out excess water (Contractile Vacuole)
 

 

Provide extra support to plant cells (Central Vacuole)

11.  What does genetic linkage mean?

               ...genes that are carried on the same chromosome, part of the same piece of DNA.


12.   If a cell has lots of golgi bodies, what particular function is it probably performing?

               ...secretion.


13.   Cells with lots of microvilli are generally doing one or both of which particular basic functions?  Microvilli increase surface area for...

Absorption

Secretion
14.   What is the purpose of a polar body?

               ...during egg cell production divisions, all of the food stays in one cell, but polar bodies are needed to remove the "extra" chromosomes that won't be in the final cell.


15.  What are two different structures that make up cytoskeleton?

Microtubules

Microfilaments

 

LONG ANSWER.

Answer any four of the following questions for Eight Points Each.
Note:
if you answer more than four, only the first four will be corrected.
You can get partial credit on these answers.


1.  Give three sets of differences between -
CILIA FLAGELLA
Smaller Larger
Always very numerous on cell Almost never more than 12 on a cell
Always highly coordinated Rarely very coordinated
Tend to move with stroke Tend to move with spin
Don't have add-on structures May have add-on structures


2.  Give three sets of differences (other than the associated genders) between -
EGG CELLS SPERM
Much larger (hold food for embryo) Much smaller
Not so many made Many more made
Must be reached by sperm Has way to get to egg cell
In production, one starting cell makes just one egg cell (and 3 polar bodies) In production, one cell used to make 4 sperm


3.  Two different things that happen during transcription in a cell. DNA strands separate
Messenger RNA made from one side of DNA
Two different things that happen during translation in a cell. Messenger RNA feeds through ribosome
Transfer RNA brings in amino acids

Transfer RNAs attach to codons on mRNA

Amino acids from tRNAs are bonded in sequence



4.  For the stages of mitosis (actual division phases), give the names of the phases in order and give one thing that happens particularly in that phase.
Prophase
Chromatin becomes visible.
Chromatin becomes chromosomes.
Nuclear envelope disappears.
Nucleolus disappears.
Spindle appears.
Spindle attaches to centromeres on chromosomes.
Chromosomes (double-stranded) are pulled into middle of cell.
Metaphase
Chromosomes line up on middle / equator of cell.
Centromeres separate, producing single-strand chromosomes.
Anaphase
Chromosomes are pulled away from each other.
Cell plate forms in plant cells.
Telophase
Chromosomes become chromatin.
Chromatin becomes invisible.
Nuclear envelope reappears.
Nucleolus reappears.
Spindle disappears.

5.   Define these terms associated with chromosome structure:
HISTONES:    Proteins that DNA is wrapped up on.

CENTROMERES:   Holds duplicate chromosomes / chromatids together.

 

CHROMATIDS:   Connected duplicate chromosomes.

 

TELOMERES:  "Caps" at ends of chromosomes.

 



6.  Given here is one side (strand) of DNA. Starting with this strand, and using the table attached to the back of the exam, show:

Starting  __________________________________________________________________________________
Strand      T    A    C    A    G    C    C    C    C    G    C    A    T    T    G    C    T    A    G    G    G    A    T    T

                 A    U    G  - U    C    G  - G    G    G  - C    G    U  - A    A    C  - G    A    U  - C    C    C  - U    A    A
                __________________________________________________________________________________
                                                                     
Messenger RNA from 1st strand

     (start)      Serine  -  Glycine  -  Arginine  -  Asparagine  -  Aspartate  -  Proline       (stop)
    ___________________________________________________________________________________________
                                                                               
Amino Acid Sequence
 


BONUS QUESTIONS.

Answer as many as you are able. Wrong answers will not result in points being lost from the main exam. You can get partial credit on these answers.

The ability to produce a particular structure turns a cancer malignant. What structure? Three Points.


Why exactly is an extra chromosome passed to an embryo very dangerous? Three Points.


What sorts of signals can "go bad" and produce cancer cells? Three Points each.


What was the really convincing evidence for the endosymbiont theory? Three Points.


Why does a progressing cancer pretty much have to reactivate its telomerase gene expression? Three Points.


What determines whether an alligator is male or female? Three Points.




HIV escape mutants have to be able to do what two things? Three Points each.

DNA proofreading works best in organisms that fill in below . Three Points.




What disease is probably caused by prions? Three Points.


 

GENE TRANSLATION TABLE

CODONS ON MESSENGER RNA


First

Letter

SECOND

LETTER

Third

Letter

U C A G
 

U

phelyalanine serine tyrosine cysteine U
phelyalanine serine tyrosine cysteine C
leucine serine STOP STOP A
leucine serine STOP tryptophan G
 

C

leucine proline histidine arginine U
leucine proline histidine arginine C
leucine proline glutamine arginine A
leucine proline glutamine arginine G
 

A

isoleucine threonine asparagine serine U
isoleucine threonine asparagine serine C
isoleucine threonine lysine arginine A
*START* /  methionine threonine lysine arginine G
 

G

valine alanine aspartate glycine U
valine alanine aspartate glycine C
valine alanine glutamate glycine A
valine alanine glutamate glycine G




 
     

 

 

 

Michael McDarby.

SC 135

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